Basically,a buffy coat is a combination ofplatelets and white blood cellsfrom the whole blood sample. The blood is concentrated using a centrifugation process collected in EDTA anticoagulant. The fraction of the anti-coagulated blood sample consisting of white blood cells and platelets is the buffy coat.
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What is a buffy coat?
What is the buffy coat? It is a concentration of leukocyte suspension. It is called buffy coat because of a buff; which is a kind of yellowish to brownish in color (buff in hue). Basically, a buffy coat is a combination of platelets and white blood cells from the whole blood sample.
What fraction of the blood sample is buffy coat?
The fraction of the anti-coagulated blood sample consisting of white blood cells and platelets is the buffy coat. Buffy coat is less than 1% of the total blood volume.
What is the difference between PBMC and buffy coat?
When peripheral whole blood is centrifuged, a white blood cell 鈥渂uffy coat鈥?layer is separated out. The buffy coat layer contains lymphocytes, monocytes, and granulocytes (Figure 1). However, when whole blood is layered on a density gradient and centrifuged, a clean PBMC layer is separated out.
What are the advantages of a buffy coat of blood?
A buffy coat is obtained and the blood is smeared. The smeared blood contains a higher number of white blood cells as opposed to using the whole blood. It improves platelet yield. It is the ideal method in meeting the emergency requirement for platelet. It greatly reduces the possibility of leukocyte contamination in platelet.