Concentration of leukocyte suspension
People also ask
What is a buffy coat made of?
Buffy Coat: Definition, Composition, Preparation and Uses. A buffy coat suspension is a concentrated leukocyte suspension. It contains most of the white blood cells and platelets from a whole blood sample. Blood stages can be concentrated using centrifugation of blood collected in EDTA anticoagulant, placed in a Wintrobe tube.
What is a buffy coat in blood work?
Basically, a buffy coat is a combination of platelets and white blood cells from the whole blood sample. The blood is concentrated using a centrifugation process collected in EDTA anticoagulant.
What is the difference between plasma and buffy coat?
Whole blood samples can be fractionated as a pretreatment to separate buffy coat, comprising white blood cells and platelets, from erythrocytes and plasma. Plasma: 55% of total Blood. Buffy Coat: Leukocytes and Platelets (1% of total blood) Erythrocytes: 45% of total blood.
What is the role of buffy coat preparation in HPC?
Buffy coat preparation is often the first step towards additional processing for T-cell depletion, tumor cell purging or cryopreservation for both marrow and blood HPC collections.