Concentration of leukocyte suspension
People also ask
Why is it called a buffy coat?
The term buffy coat arose from the fact that the suspension has a color (yellowish beige) that is similar to buff. Buffy coats primarily contain white blood cells and platelets when separated from the whole blood sample via centrifugation.
What is buffy coat blood banking?
The term 鈥渂uffy coat鈥?might make you think of a shiny car wax, but in the world of blood banking, buffy coat refers to the white layer between red blood cells and plasma in a unit of whole blood after it has been spun down in a centrifuge. The buffy coat contains white blood cells, the soldiers of the immune system.
What is the difference between PBMC and buffy coat?
When peripheral whole blood is centrifuged, a white blood cell 鈥渂uffy coat鈥?layer is separated out. The buffy coat layer contains lymphocytes, monocytes, and granulocytes (Figure 1). However, when whole blood is layered on a density gradient and centrifuged, a clean PBMC layer is separated out.
What fraction of the blood sample is buffy coat?
The fraction of the anti-coagulated blood sample consisting of white blood cells and platelets is the buffy coat. Buffy coat is less than 1% of the total blood volume.