what does a buffy coat contain

Best answer Basically,a buffy coat is a combination ofplatelets and white blood cellsfrom the whole blood sample. The blood is concentrated using a centrifugation process collected in EDTA anticoagulant. The fraction of the anti-coagulated blood sam

Best answer


Basically,a buffy coat is a combination ofplatelets and white blood cellsfrom the whole blood sample. The blood is concentrated using a centrifugation process collected in EDTA anticoagulant. The fraction of the anti-coagulated blood sample consisting of white blood cells and platelets is the buffy coat.

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  • What is the buffy coat?

  • What is the Buffy Coat? The buffy coat accounts for less than 1 percent of a whole blood sample, yet it contains the majority of white blood cells (WBC) and platelets. In fact, leukocytes can be 10-20X more concentrated in the buffy coat than in whole blood.

  • What is the buffy coat layer of the blood?

  • Buffy Coat: Leukocytes and Platelets (1% of total blood) The buffy coat layer, containing most of the white blood cells and platelets, is situated between the plasma and erythrocytes. The buffy coat is used to extract DNA from the blood of mammals (since mammalian red blood cells are anucleate and do not contain DNA).

  • What is the difference between plasma and buffy coat?

  • Whole blood samples can be fractionated as a pretreatment to separate buffy coat, comprising white blood cells and platelets, from erythrocytes and plasma. Plasma: 55% of total Blood. Buffy Coat: Leukocytes and Platelets (1% of total blood) Erythrocytes: 45% of total blood.

  • What are buffy coat leukocytes and why are they important?

  • In fact, leukocytes can be 10-20X more concentrated in the buffy coat than in whole blood. Therefore these samples are highly valuable for researchers and healthcare professionals who require WBC and platelets for studies or treatments.

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